Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

2.9K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
2.9K
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

6.1K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
6.1K
Drug Discovery: Overview01:26

Drug Discovery: Overview

7.6K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expanding the Clinical and Genetic Landscape of UQCRC2-related Mitochondrial Complex III Deficiency: A Case Report and Literature Review.

Current pediatric reviews·2026
Same author

Screening for Cervical Cancer: A Recommendation From the Women's Preventive Services Initiative.

Obstetrics and gynecology·2026
Same author

A co-created randomised controlled feasibility trial of a peer-led complex oral health intervention in UK secondary schools: the RAISED In Yorkshire RiY study protocol.

Pilot and feasibility studies·2026
Same author

Discovery of Clinical Candidate IAG933, a Potent YAP-TEAD PPI Disrupter.

Journal of medicinal chemistry·2026
Same author

Re: Self-Collected Vaginal Specimens at Home for HPV Testing: Recommendations From the Enduring Guidelines Committee.

Journal of lower genital tract disease·2026
Same author

Development of a p62 biodegrader for autophagy targeted degradation.

Nature communications·2025

Related Experiment Video

Updated: Jun 12, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.6K

Oxadiazolines as Photoreleasable Labels for Drug Target Identification.

Corentin Bon1, Benedikt Goretzki2, Marie Flamme3

  • 1Global Discovery Chemistry, Novartis Biomedical Research, Novartis Pharma AG, Basel 4056, Switzerland.

Journal of the American Chemical Society
|September 17, 2024
PubMed
Summary

Oxadiazolines offer a novel photoaffinity labeling method, overcoming limitations of traditional diazirines. These labels enable covalent modification of proteins in vitro and in cells with improved chemical compatibility.

More Related Videos

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.4K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.2K

Related Experiment Videos

Last Updated: Jun 12, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.6K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.4K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.2K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Organic Chemistry

Background:

  • Photoaffinity labeling is crucial for studying molecular interactions.
  • Traditional labels like diazirines have limitations in chemical compatibility and stability.
  • A need exists for improved photoactivatable labeling tools.

Purpose of the Study:

  • Introduce oxadiazolines as a new class of photoactivatable labels.
  • Demonstrate the utility of oxadiazolines for in vitro and in cellulo labeling.
  • Compare oxadiazoline performance with established diazirine labels.

Main Methods:

  • Synthesize oxadiazolines from ketone precursors.
  • Utilize UV light (302-330 nm) for photoactivation.
  • Assess covalent modification of nucleophilic amino acid residues in proteins.
  • Evaluate compatibility with diverse organic reactions and functional groups.
  • Perform in vitro and in cellulo labeling experiments.

Main Results:

  • Oxadiazolines are synthesized and photoactivated to generate reactive diazo intermediates.
  • These intermediates efficiently form covalent bonds with proteins.
  • Oxadiazolines exhibit broad compatibility with various chemical conditions.
  • In vitro and in cellulo labeling results are comparable to diazirines.
  • Preparation of known inhibitors with oxadiazoline functionality did not impact binding affinity.

Conclusions:

  • Oxadiazolines represent a versatile and complementary alternative to diazirine photoaffinity labels.
  • Their enhanced chemical stability and compatibility expand labeling possibilities.
  • Oxadiazolines are effective for studying ligand-protein and protein-protein interactions in biological systems.