A protectant for Lactobacillus rhamnosus based on whey protein isolate and isomalt: Stress resistance and underlying...

Probiotics are exposed to a variety of abiotic and biotic stresses during food fermentation and production, such as acidity, heat, osmolality, and oxidation, which affect their metabolic activity and efficiency. Therefore, it is essential to develop new protective agents to maintain the activity and stability of probiotics. This study introduces a new protectant, spray-dried whey protein isolate (WPI) and isomaltose (ISO). We evaluated the effects of four WPI-ISO ratios (1:0, 2:1, 1:1, 1:2) on the physical properties, including moisture content, water activity (aw), wettability, and glass transition temperature. In addition, we evaluated the environmental tolerance of Lactobacillus rhamnosus to different WPI-ISO ratios under thermal, storage, and simulated gastrointestinal conditions. The results showed that the moisture content (< 7 %) and water activity (< 0.3) of the protectant and probiotic powders met storage stability requirements. The moisture content, water activity, wettability index (WI), and glass transition temperature decreased significantly with the addition of isomalt, thereby improving the pressure resistance of L. rhamnosus through the synergistic effect of WPI and ISO. The WPI-ISO protectant not only improved the environmental tolerance and wettability of probiotics by reducing the moisture content and water activity but also significantly improved the survival rate of L. rhamnosus under various stress conditions such as high temperature and gastrointestinal environment. L. rhamnosus maintains good activity with a viable bacterial count of over 9 lg CFU/g after 90 days of storage, demonstrating effective protection against the environment stress. This study provides a promising new strategy to improve the stability of probiotics in the food industry.