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Hageman factor in experimental nephrotoxic nephritis in the rabbit
Laboratory Investigation; a Journal of Technical Methods and Pathology
|September 1, 1985
Summary
The Hageman factor (HF) system may contribute to kidney damage by passively crossing a damaged glomerular filter and becoming activated in Bowman's space or tubules during nephrotoxic nephritis.
Area of Science:
- Nephrology
- Immunology
- Hematology
Background:
- Glomerular damage in nephrotoxic nephritis involves inflammation and fibrin deposition.
- The role of the Hageman factor (HF) system in this process is not fully understood.
Purpose of the Study:
- To investigate the potential contribution of the Hageman factor (HF) system to glomerular damage in a rabbit model of nephrotoxic nephritis.
Main Methods:
- Developed a rabbit model of nephrotoxic nephritis.
- Purified rabbit HF and generated anti-HF IgG for detection.
- Utilized immunofluorescence and radiolabeled HF (125I-HF) to track HF presence and activation.
Main Results:
- HF was not detected in the glomerular capillary wall but was present in Bowman's space and tubules.
- Activated HF fragments and HF-dependent procoagulant activity were found in the urine of nephritic rabbits.
- Increased turnover of HF was observed, though less than albumin turnover.
Conclusions:
- HF appears to passively cross the damaged glomerular filter.
- HF activation may occur in Bowman's space or tubular fluid, contributing to glomerular injury in this nephritis model.