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In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
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  11. Mechanism Study Of Yangjing Zhongyu Decoction On Regulating Mitochondrial Dynamics Of Ovarian Granular Cells And Improving Diminished Ovarian Reserve

Mechanism study of YangJing ZhongYu decoction on regulating mitochondrial dynamics of ovarian granular cells and improving diminished ovarian reserve

Ping Li1, Jilin Kuang2

  • 1Department of Gynecology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, 233 CAI 'e North Road, Kaifu District, Changsha, 410005, Hunan, China.

Journal of Ovarian Research
|September 17, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Yangjing Zhongyu Decoction (YJZYD) improves diminished ovarian reserve (DOR) by enhancing granulosa cell (GC) proliferation and mitochondrial fusion. This traditional Chinese medicine treatment activates the MAPK/ERK pathway, offering a new therapeutic avenue for DOR.

Area of Science:

  • Reproductive endocrinology and cell biology.
  • Investigating traditional Chinese medicine's impact on female fertility.
  • Mitochondrial dynamics in ovarian function.

Background:

  • Diminished ovarian reserve (DOR) is a significant cause of female infertility.
  • Current treatments for DOR have limitations.
  • Understanding the cellular mechanisms underlying DOR is crucial for developing effective therapies.

Purpose of the Study:

  • To explore the mechanism of Yangjing Zhongyu Decoction (YJZYD) in regulating mitochondrial dynamics of ovarian granulosa cells (GCs) to improve DOR.
  • To investigate the role of the MAPK/ERK pathway in YJZYD's therapeutic effects.

Main Methods:

  • Clinical assessment of DOR patients treated with YJZYD, measuring ovarian volume (OV), antral follicle count (AFC), and endometrial thickness (EMT).
Keywords:
ApoptosisDiminished ovarian reserveMitochondrial dynamicsOvarian granulosa cells

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  • Animal study using C57BL/6 mice induced with cyclophosphamide (Cy) and treated with YJZYD, assessing hormonal levels (AMH, FSH, LH, E2) and ovarian morphology.
  • In vitro study using ovarian GCs (KGN) treated with 4-Hydroperoxy-Cyclophosphamide (4-HC) and MAPK/ERK pathway modulators, evaluating proliferation, apoptosis, and mitochondrial dynamics.

    • Main Results:

    • YJZYD treatment in DOR patients led to improved reproductive parameters (increased AMH, E2, OV, AFC, EMT; decreased FSH, LH).
    • In DOR mice, YJZYD reversed adverse effects of Cy, including hormonal imbalances, reduced follicle count, and GCs disarray.
    • YJZYD promoted GC proliferation, enhanced mitochondrial fusion, inhibited apoptosis, and reduced mitochondrial fission in vitro, mediated by the MAPK/ERK pathway.

    Conclusions:

    • YJZYD effectively improves DOR by promoting ovarian GC proliferation and mitochondrial fusion while suppressing apoptosis and mitochondrial fission.
    • The therapeutic effects of YJZYD in DOR are achieved through the activation of the MAPK/ERK pathway.
    • YJZYD demonstrates significant potential for clinical application in treating diminished ovarian reserve.
    Proliferation
    YangJing zhongyu decoction