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Some interleukin-3 dependent mast-cell lines also respond to interleukin-2
Lymphokine Research
|January 1, 1985
Summary
The 32D cl-23 cell line, dependent on interleukin-3 (IL-3), also responds to interleukin-2 (IL-2). This response is mediated by a receptor similar to that found on T cells, as shown by antibody blocking experiments.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- The 32D cl-23 cell line is a murine haemopoietic stem cell dependent on interleukin-3 (IL-3) for growth.
- This cell line expresses Lyt 1 antigen and is alcian blue positive.
Purpose of the Study:
- To investigate the response of the 32D cl-23 cell line to interleukin-2 (IL-2).
- To characterize the interleukin-2 receptor on 32D cl-23 cells and compare it to the receptor on murine T cells.
Main Methods:
- Utilizing the 32D cl-23 cell line and other basophil cell lines (32D cl-5, Rotundi) and mast cells (P cells).
- Assessing cellular response to interleukin-2 (IL-2) and interleukin-3 (IL-3).
- Employing antibodies against the murine interleukin-2 receptor to block 3H-thymidine incorporation.
Main Results:
- The 32D cl-23 cell line demonstrated a response to interleukin-2 (IL-2).
- Antibodies against the murine interleukin-2 receptor inhibited 3H-thymidine incorporation in 32D cl-23 cells cultured with IL-2.
- These antibodies had no effect on 32D cl-23 cells cultured with IL-3.
- Similar IL-2 responsiveness was observed in the 32D cl-5 cell line, but not in Rotundi or P cells.
Conclusions:
- The 32D cl-23 cell line possesses a functional interleukin-2 (IL-2) receptor.
- The IL-2 receptor on 32D cl-23 cells appears similar to the IL-2 receptor found on murine T cells.
- Cell line-specific differences exist in IL-2 receptor expression and function among myeloid and mast cell populations.