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Interferon gamma: more than a MAF.

P M Peters, L P Svedersky, M R Shalaby

    Lymphokine Research
    |January 1, 1985
    PubMed
    Summary
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    Recombinant gamma interferon (rIFN-gamma) clarifies lymphokine functions. This study shows rIFN-gamma is a key macrophage activating factor (MAF) and regulates T cell growth.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biotechnology

    Background:

    • Defining lymphokine activities was challenging due to contaminants in natural preparations.
    • Recombinant DNA technology has enabled precise study of lymphokine properties.

    Purpose of the Study:

    • To elucidate the immunoregulatory functions of lymphokines, particularly gamma interferon (IFN-gamma).
    • To compare macrophage activating factor (MAF) properties with natural and recombinant IFN-gamma (rIFN-gamma).

    Main Methods:

    • Utilized recombinant DNA technology to produce purified rIFN-gamma.
    • Compared the biological activities of rIFN-gamma with natural MAF and IFN-gamma.
    • Investigated the effects of rIFN-gamma on interleukin 2 (IL-2)-dependent cytotoxic T cell lines.

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    Main Results:

    • Recombinant gamma interferon (rIFN-gamma) mediates various immuno-regulatory functions, including natural killer cell activation and macrophage activation.
    • Evidence strongly suggests that IFN-gamma is a major MAF.
    • rIFN-gamma demonstrates the ability to regulate the growth of specific IL-2-dependent cytotoxic T cell lines.

    Conclusions:

    • Gamma interferon (IFN-gamma) is a pivotal lymphokine with diverse immunoregulatory roles.
    • rIFN-gamma serves as a potent MAF, impacting macrophage function.
    • IFN-gamma possesses additional functions, including the regulation of cytotoxic T cell proliferation.