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Harnessing the Antioxidative Potential of Dental Pulp Stem Cell-Conditioned Medium in Photopolymerized GelMA Hydrogels

  • 0Center of Translational Oral Research, Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
Biomaterials Research +

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September 18, 2024

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September 18, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study investigated Gelatin methacryloyl (GelMA) photocrosslinking effects on stem cells. Dental pulp stem cell conditioned medium (DPSC-CM) was used to mitigate oxidative stress and improve cell health and biocompatibility.

Area Of Science

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background

  • Gelatin methacryloyl (GelMA) is a widely used biomaterial for tissue scaffolding due to its favorable properties.
  • The biological impacts of GelMA photocrosslinking on mesenchymal stem cells, particularly oxidative stress, are not fully understood.
  • Strategies for mitigating adverse effects during GelMA fabrication require further investigation.

Purpose Of The Study

  • To comprehensively analyze the biological effects of GelMA photocrosslinking on mesenchymal stem cells, focusing on oxidative stress.
  • To develop and evaluate a mitigation strategy using dental pulp stem cell conditioned medium (DPSC-CM).

Main Methods

  • Utilized Good Manufacturing Practice (GMP) grade GelMA for experiments.
  • Photocrosslinked GelMA hydrogels with and without DPSC-CM.
  • Assessed cellular responses including oxidative stress, DNA repair, inflammatory pathways, cell viability, proliferation, motility, and osteogenic differentiation.
  • Evaluated biocompatibility using the chicken chorioallantoic membrane (CAM) assay.

Main Results

  • DPSC-CM addition upregulated antioxidant pathways (PRDX, SOD1) and DNA repair, while controls showed inflammatory signaling.
  • DPSC-CM significantly reduced cellular oxidation and stress responses induced by photocrosslinking.
  • Improved cell viability, growth, motility, osteogenic differentiation, and reduced apoptosis/senescence were observed with DPSC-CM.
  • CAM assay demonstrated prevention of vascular disruption by DPSC-CM, indicating enhanced biocompatibility.

Conclusions

  • DPSC-CM exhibits potent antioxidative properties that effectively mitigate the negative biological impacts of GelMA photocrosslinking.
  • The use of DPSC-CM represents a promising strategy to enhance the biocompatibility and therapeutic potential of GelMA-based biomaterials.