DNAJC3-AS1 Is Associated with Proliferation, Metastasis, and Poor Prognosis of Breast Cancer
View abstract on PubMed
Summary
This summary is machine-generated.Long noncoding RNA DNAJC3-AS1 (DNAJC3-AS1) is elevated in breast cancer (BC) and serves as a diagnostic and prognostic biomarker. High DNAJC3-AS1 expression correlates with poor survival and advanced disease, suggesting its role in tumor progression.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
- DNAJC3-AS1 is a newly identified lncRNA with potential involvement in tumorigenesis.
Purpose Of The Study
- To investigate the diagnostic and prognostic value of DNAJC3-AS1 expression in breast cancer (BC).
- To explore the functional role of DNAJC3-AS1 in BC progression.
Main Methods
- Quantitative reverse transcription PCR (qRT-PCR) to measure DNAJC3-AS1 expression in 170 BC tissues and matched normal samples.
- Receiver-operating characteristic (ROC) curve analysis for diagnostic accuracy.
- Correlation analysis with clinicopathological features and survival data.
- Cell proliferation (CCK-8, colony formation) and migration/invasion (Transwell) assays.
- Western blot for epithelial-mesenchymal transition (EMT) markers.
Main Results
- DNAJC3-AS1 expression was significantly higher in BC tissues than in adjacent normal tissues (p < 0.01).
- DNAJC3-AS1 demonstrated good diagnostic accuracy (AUC = 0.7457, p < 0.001).
- High DNAJC3-AS1 expression correlated with lymph node metastasis (p = 0.010) and advanced clinical stage (p = 0.023).
- Elevated DNAJC3-AS1 predicted shorter overall survival (p = 0.0067) and disease-free survival (p < 0.0001).
- Multivariate analysis confirmed DNAJC3-AS1 as an independent prognostic factor.
- Silencing DNAJC3-AS1 suppressed BC cell proliferation, metastasis, and EMT.
Conclusions
- DNAJC3-AS1 is a promising diagnostic biomarker for breast cancer.
- DNAJC3-AS1 serves as a significant independent prognostic factor for BC patients.
- DNAJC3-AS1 plays a crucial role in promoting BC cell proliferation, metastasis, and EMT.
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