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Related Concept Videos

Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

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Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
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COPD: Pathogenesis and Clinical Features01:20

COPD: Pathogenesis and Clinical Features

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Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
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Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies01:27

Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies

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Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History
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Chronic Obstructive Pulmonary Disease-I: Introduction01:20

Chronic Obstructive Pulmonary Disease-I: Introduction

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Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.
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  11. Construction Of Circrna-mediated Cerna Network And Immunoassay For Investigating Pathogenesis Of Copd

Construction of circRNA-mediated ceRNA network and immunoassay for investigating pathogenesis of COPD

Ting Yang1,2, Wenya Xu3, Jie Zhao3

  • 1Department of General Practice, Hainan Affiliated Hospital of Hainan Medical University, Hainan General Hospital, Haikou, Hainan, China.

Frontiers in Genetics
|September 18, 2024

Related Experiment Videos

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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In vivo Evaluation of Mucociliary Clearance in Mice
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In vivo Evaluation of Mucociliary Clearance in Mice

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Quantification of Circular RNAs Using Digital Droplet PCR
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Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

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View abstract on PubMed

Summary
This summary is machine-generated.

This study identified the circDTL-hsa-miR-330-3p-CCL20 network as a key mechanism in chronic obstructive pulmonary disease (COPD). This finding offers new therapeutic targets for COPD treatment.

Area of Science:

  • Molecular Biology
  • Genomics
  • Immunology

Background:

  • Chronic obstructive pulmonary disease (COPD) is a major global health burden, causing significant death and disability.
  • Understanding the complex molecular mechanisms underlying COPD is crucial for developing effective treatments.

Purpose of the Study:

  • To elucidate novel molecular targets and mechanisms in COPD pathogenesis.
  • To construct and analyze competitive endogenous RNA (ceRNA) networks in COPD.

Main Methods:

  • Differential gene expression analysis of COPD datasets (GSE38974, GSE106986).
  • Construction of protein-protein interaction (PPI), mRNA-miRNA co-expression, and ceRNA networks using Cytoscape.
  • Screening of immune infiltrating cells and hub genes using CIBERSORT and Lasso models.
Keywords:
CCL20chronic obstructive pulmonary diseasecircDTLcompetitive endogenous RNA

Related Experiment Videos

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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06:35

In vivo Evaluation of Mucociliary Clearance in Mice

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Quantification of Circular RNAs Using Digital Droplet PCR
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  • In vitro validation of ceRNA network factor expression in COPD cell models via RT-qPCR.

    • Main Results:

    • Identified 852 differentially expressed genes, including seven hub genes.
    • Validated CCL20 as a crucial hub gene through network analysis and ROC curves.
    • Constructed the circDTL-hsa-miR-330-3p-CCL20 ceRNA network.
    • Demonstrated CCL20's role in immune cell migration via chemokines and confirmed altered expression of circDTL, hsa-miR-330-3p, and CCL20 in COPD cell models.

    Conclusions:

    • The circDTL-hsa-miR-330-3p-CCL20 network provides insights into COPD molecular mechanisms.
    • This network represents a potential therapeutic target for developing novel COPD treatment strategies.
    hsa-miR-330-3p