Robust and low-cost open-source device for detecting infectious microorganisms by loop-mediated isothermal...

Area of Science:

• Molecular Biology
• Biotechnology
• Medical Diagnostics

Background:

• Loop-Mediated Isothermal Amplification (LAMP) offers rapid detection of infectious agents.
• LAMP requires less complex equipment than conventional PCR, making it suitable for resource-limited settings.
• Existing LAMP methods may face challenges in stability and accessibility in Low- and Middle-Income Countries (LMICs).

Purpose of the Study:

• To develop and validate a low-energy, Arduino-controlled LAMP thermoblock system.
• To assess the system's performance under challenging environmental conditions and low power availability.
• To create a cost-effective, portable detection device for field use in LMICs.

Main Methods:

• Designed and tested Arduino-controlled isothermal thermoblocks for cell lysis and DNA amplification.
• Evaluated thermoblock temperature stability and energy consumption using battery power at low ambient temperatures (down to 5°C).
• Developed a low-cost fluorescent reader using LEDs and filters for LAMP reaction detection.

Main Results:

• Thermoblocks demonstrated high temperature stability (<0.8°C) even when battery-powered at 5°C.
• Energy requirements were quantified for initial heating (4.1 A·h) and continuous operation (2.4 A·h/h).
• The complete LAMP device utilizes readily available components, enabling autonomous operation with a car battery.

Conclusions:

• The developed Arduino-controlled LAMP system is robust and energy-efficient for field applications.
• This technology can significantly enhance infectious disease diagnostics in LMICs, even in remote or challenging environments.
• The system's low cost and portability make it a viable solution for decentralized healthcare and field hospitals.