Troxerutin Delays Skin Keratinocyte Senescence Induced by Ionizing Radiation Both In Vitro and In Vivo
Juping Chen1,2, Jinghui Yang1,2, Jiang Ma1,2
1Department of the Central Laboratory, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Backgrounds:
With the increasing demand for beauty and a healthy lifespan, studies regarding anti-skin aging have drawn much more attention than ever before. Skin cellular senescence, the primary cause of skin aging, is characterized by a cell cycle arrest in proliferating cells along with a senescence-associated secretory phenotype (SASP), which can be triggered by various internal or external stimuli.
Aims:
Recent studies have made significant progress in the fields of anti-senescence and anti-aging. However, little is known about the roles and functions of natural compounds, particularly flavonoids, in skin cellular senescence studies.
Methods:
In this study, using strategies including ionizing radiation (IR), senescence-associated β galactosidase assay (SA-β-Gal), immunofluorescence (IF), flow cytometry, PCR array, as well as in vivo experiments, we investigated the effects and roles of troxerutin (Trx), a natural flavonoid, in skin keratinocyte senescence.
Results:
We found that Trx delays skin keratinocyte senescence induced by IR. Mechanistically, Trx protects the skin keratinocyte cells from senescence by alleviating reactive oxygen species (ROS) accumulation, mitochondrial dysfunction, and DNA damage caused by IR. In addition, Trx was also proved to relieve skin senescence and SASP secretion in vivo induced by IR stimulation.
Conclusions:
Altogether, our findings pointed to a new function of Trx in delaying stress-induced skin keratinocyte senescence, and should thus provide theoretical foundations for exploring novel strategies against skin aging.
Insights
Troxerutin (Trx), a natural flavonoid, delays skin aging by preventing cellular senescence. This compound alleviates oxidative stress, mitochondrial dysfunction, and DNA damage, offering new anti-aging strategies.
Area of Science:
- Gerontology
- Dermatology
- Biochemistry
Background:
- Skin aging is a growing concern, with cellular senescence as a key driver.
- Cellular senescence involves cell cycle arrest and the senescence-associated secretory phenotype (SASP).
- Natural compounds, especially flavonoids, are increasingly studied for anti-aging potential.
Purpose of the Study:
- To investigate the effects of troxerutin (Trx), a natural flavonoid, on skin keratinocyte senescence.
- To explore the mechanisms by which Trx influences stress-induced skin aging.
Main Methods:
- Ionizing radiation (IR) was used to induce skin keratinocyte senescence.
- Assays included senescence-associated β-galactosidase (SA-β-Gal), immunofluorescence (IF), and flow cytometry.
- In vivo experiments and PCR arrays were employed to assess Trx's effects.
Main Results:
- Troxerutin (Trx) effectively delays IR-induced skin keratinocyte senescence.
- Trx mitigates senescence by reducing reactive oxygen species (ROS) accumulation, mitochondrial dysfunction, and DNA damage.
- In vivo studies confirmed Trx's ability to alleviate skin aging and SASP secretion.
Conclusions:
- Troxerutin (Trx) demonstrates a novel function in delaying stress-induced skin keratinocyte senescence.
- These findings support Trx as a potential therapeutic agent for combating skin aging.
- Provides a theoretical basis for developing new anti-aging strategies targeting cellular senescence.
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