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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 12, 2025

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

High-throughput single telomere analysis using DNA microarray and fluorescent in situ hybridization.

Yun-Ling Zheng¹, Xingjia Wu¹, Madeline Williams¹

¹Cancer Prevention and Control Program, Department of Oncology, Georgetown University Medical Center, Georgetown University, Washington, DC 20057, USA.

Nucleic Acids Research

|September 18, 2024

Summary

New DNA-array-FISH technology precisely measures single telomere lengths. This high-throughput method aids in understanding telomere dynamics and their link to diseases, revealing complex health associations.

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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

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Last Updated: Jun 12, 2025

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

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Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Published on: March 4, 2021

Area of Science:

Genetics
Molecular Biology
Biotechnology

Background:

The human telomere system is dynamic, with average telomere lengths (aTL) linked to cancer and mortality risks.
Assessing telomere length (TL) distributions using single telomere analysis is crucial for understanding human health.
Existing methods may not offer the throughput or precision needed for comprehensive TL analysis.

Purpose of the Study:

To develop and validate a novel, high-throughput method for measuring the base-pair (bp) lengths of single telomeres.
To enable in-depth analysis of telomere dynamics and their relationship with age-related diseases.
To provide a precise tool for assessing telomere length distributions.

Main Methods:

Development of a DNA microarray and telomere fluorescent in situ hybridization (DNA-array-FISH) approach.
Measurement of approximately 32,000 single telomeres per DNA sample using one microarray chip for 96 samples.
Computation of various telomere parameters, including aTL and the frequency of short/long telomeres.

Main Results:

The DNA-array-FISH method demonstrated high measurement precision, with aTL coefficients of variation ranging from 1.37% to 3.98%.
High correlation coefficients (r=0.91-1.00) were observed for aTL in repeated measurements.
aTLs measured by DNA-array-FISH accurately predicted aTLs measured by terminal restriction fragment (TRF) analysis (r=0.87-0.99).

Conclusions:

A new, accurate, and high-throughput method for measuring single telomere lengths in base pairs has been successfully developed.
The large volume of single telomere data generated offers significant potential for detailed analysis of telomere dynamics.
This method facilitates a deeper understanding of the complex relationship between telomere length and age-related diseases.