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Related Experiment Video

Updated: Jun 9, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Engineered chimeric receptors for dissecting interferon signaling.

Aaron E Lin^1,2, Emily V Mesev¹, Jared E Toettcher^1,3

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.

Journal of Virology

|September 18, 2024

Summary

Scientists engineered new chimeric receptors to understand how cells respond to different types of interferons (IFNs). This research reveals genetic factors influencing type I and type III IFN signaling pathways.

Keywords:

STAT signaling flavivirus innate immunity interferons virus-host interactions

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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

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Last Updated: Jun 9, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

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Published on: March 24, 2015

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Area of Science:

Immunology
Molecular Biology
Genetics

Background:

Interferons (IFNs) are crucial for immune responses but how cells interpret diverse IFN signals is not fully understood.
Understanding differential signaling is key to developing effective therapies for IFN-related diseases.

Purpose of the Study:

To investigate the mechanisms by which cells decode varying type I (IFN-α/β) and type III (IFN-λ) interferon stimuli.
To explore the utility of novel erythropoietin receptor-IFN chimeric receptors in dissecting IFN signaling pathways.

Main Methods:

Engineering of novel erythropoietin receptor-IFN chimeric receptors.
Utilizing these chimeric receptors to uncover differential genetic determinants of IFN signaling.

Main Results:

The study identified distinct genetic factors that differentiate the cellular responses to type I and type III interferons.
Demonstrated the effectiveness of engineered chimeric receptors in revealing these signaling differences.

Conclusions:

Engineered synthetic receptors offer a powerful tool for studying real-time cytokine signaling dynamics.
This approach can be expanded for in vivo studies to further elucidate interferon response mechanisms.