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Related Concept Videos

Microbe-Plant Interactions01:09

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

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Related Experiment Video

Updated: Jun 6, 2026

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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Transcriptomic profiling reveals the complex interaction between a bipartite begomovirus and a cucurbitaceous host

Wen-Ze He1, Li Zhao2, Kai Sun3

  • 1Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang A&F University, Hangzhou, 311300, China.

BMC Genomics
|September 18, 2024
PubMed
Summary

Squash leaf curl China virus (SLCCNV) infection in zucchini suppresses photosynthesis while upregulating plant defense and MAPK signaling pathways. This reveals complex bipartite begomovirus-plant interactions in cucurbits.

Keywords:
Cucurbita pepoBipartite begomovirusPlant-virus interactionSquash leaf curl China virusTranscriptional reprograming

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Area of Science:

  • Plant pathology
  • Molecular biology
  • Genomics

Background:

  • Begomoviruses significantly impact crop production by altering plant processes.
  • Bipartite begomovirus-plant interactions, especially in cucurbits, are less understood than monopartite begomovirus interactions.

Purpose of the Study:

  • To investigate the transcriptomic changes in zucchini plants infected with the bipartite begomovirus, squash leaf curl China virus (SLCCNV).
  • To elucidate the molecular mechanisms underlying the interaction between SLCCNV and its cucurbit host.

Main Methods:

  • Transcriptomic profiling of zucchini plants upon SLCCNV infection.
  • Identification and analysis of differentially-expressed genes (DEGs) using KEGG enrichment.
  • Validation of DEG expression using quantitative reverse transcription PCR (qRT-PCR).

Main Results:

  • Identified 2275 DEGs, with 1310 upregulated and 965 downregulated.
  • Enrichment analysis revealed significant alterations in primary and secondary metabolism pathways.
  • Photosynthesis pathway genes were downregulated, while Plant-pathogen interaction and MAPK signaling pathways showed predominantly upregulated DEGs.

Conclusions:

  • SLCCNV actively modulates cucurbit host responses by suppressing energy conversion (photosynthesis) and activating immune responses.
  • This study provides novel insights into bipartite begomovirus-cucurbit interactions and broader virus-plant molecular dialogues.