Calibration-Free and Highly Sensitive Potentiometric Enzyme-Based Biosensors

Area of Science:

• Biosensor technology
• Biomedical engineering
• Analytical chemistry

Background:

• Enzyme-based amperometric biosensors are crucial for healthcare but require enhanced sensitivity and usability.
• Existing biosensors face challenges with external influences like temperature and pH, necessitating frequent calibration.

Purpose of the Study:

• To develop and validate enzyme-based glucose and lactate sensors using a novel time-derivative of potential (dOCP/dt) method.
• To demonstrate the proportionality of dOCP/dt values to substrate concentration.
• To achieve calibration-free biosensor operation.

Main Methods:

• Development of enzyme-based glucose and lactate sensors employing the dOCP/dt method.
• Theoretical and experimental validation of the dOCP/dt method's proportionality to substrate concentration.
• Investigation of electrode size independence and elimination of external influences.

Main Results:

• Achieved high sensitivity in biosensors irrespective of electrode size.
• Demonstrated that dOCP/dt values are directly proportional to substrate concentration.
• Successfully eliminated external influences (temperature, pH) by relying on substrate diffusion-controlled reaction rates.

Conclusions:

• The dOCP/dt method offers a pathway to highly sensitive and calibration-free enzyme-based biosensors.
• This approach significantly enhances the practicality and usability of biosensors for healthcare applications.
• The developed biosensors provide reliable measurements independent of environmental variations.