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Published on: June 3, 2019
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Nanopore-based sensors for DNA sequencing: a review
Jiangtao Wei1, Hao Hong1,2, Xing Wang1
1School of Integrated Circuits, Tsinghua University, Beijing 100084, China. liuzw@tsinghua.edu.cn.
Nanoscale
|September 19, 2024
Summary
Nanopore sensors offer promising deoxyribonucleic acid (DNA) sequencing capabilities. Nanopore field-effect transistor (FET) sensors provide high signal-to-noise ratios and bandwidth, overcoming limitations of traditional solid-state nanopore (SSN) technologies for enhanced biomolecule detection.
Area of Science:
- Biophysics
- Nanoscience
- Biotechnology
Background:
- Nanopore sensors detect biomolecular translocation events by monitoring electrical current variations.
- Traditional solid-state nanopore (SSN) technologies face challenges with signal loss due to bandwidth and noise limitations.
- Novel detection mechanisms like optical, tunneling current, and nanopore field-effect transistor (FET) detection have been developed.
Purpose of the Study:
- To review the principles, history, applications, and fabrication of traditional SSNs.
- To introduce and emphasize nanopore FET sensors as an advanced detection mechanism.
- To analyze the advantages and challenges of SSNs and nanopore FET sensors for DNA sequencing.
Main Methods:
- Elucidation of fundamental detection principles for traditional SSNs.
- Introduction of novel detection mechanisms, focusing on nanopore FET sensors.
- Comprehensive analysis of fabrication techniques, detection principles, and challenges.
Main Results:
- Nanopore FET sensors exhibit superior signal-to-noise ratio (SNR) and high bandwidth capabilities.
- These sensors overcome limitations of traditional SSN technologies.
- Advancements pave the way for improved biomolecule detection and DNA sequencing.
Conclusions:
- Nanopore FET sensors represent a significant advancement in biomolecule detection and DNA sequencing.
- Further development of nanopore FET sensors is crucial for overcoming current challenges.
- This review provides insights into fabrication, detection, and future trends in nanopore FET sensor technology.
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