Related Experiment Video

Updated: Aug 17, 2026

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

Exotoxin A from various Pseudomonas aeruginosa cultures: in vitro activity measured by enzyme-linked immunosorbent

A R Ogaard, S J Cryz, B P Berdal

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology

|June 1, 1985

Abstract:

An enzyme-linked immunosorbent assay (ELISA) for the detection of Pseudomonas aeruginosa exotoxin A (toxin A) was developed. The level of toxin A produced by fresh P. aeruginosa isolates was compared to the level of toxin A produced by twelve-months-old subcultures derived from the same strains. Results obtained with the ELISA showed that toxin A was produced in similar amounts both by the fresh isolates and the subcultures. The production of toxin A seems to represent a stable property of the assayed P. aeruginosa strains. This is in contrast to other exoproducts from the same strains, such as extracellular proteinases. Some proteinases were produced at very different levels when freshly isolated P. aeruginosa cultures were compared with their twelve-months-old counterparts, confirming earlier reports in this respect.

More Related Videos

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Published on: July 12, 2018

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Published on: June 24, 2019

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Articles linked to this work by shared authors, journal, and citation graph.

Chlamydophila pneumoniae diagnostics: importance of methodology in relation to timing of sampling.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2008

Unusual persistence in healthy volunteers and ill patients of hyperimmune immunoglobulin directed against multiple Pseudomonas O-chain and Klebsiella serotypes after intravenous infusion.

Vaccine·2001

Immunologic responses of HIV-1-infected study subjects to immunization with a mixture of peptide protein derivative-V3 loop peptide conjugates.

Journal of acquired immune deficiency syndromes (1999)·2000

Field detection of Francisella tularensis.

Scandinavian journal of infectious diseases·2000

Local and systemic immune responses to combined vibrio cholerae CVD103-HgR and salmonella typhi ty21a live oral vaccines after primary immunization and reimmunization.

Vaccine·2000

Elevated levels of maternal anti-tetanus toxin antibodies do not suppress the immune response to a Haemophilus influenzae type b polyribosylphosphate-tetanus toxoid conjugate vaccine.

Bulletin of the World Health Organization·2000

See all related articles