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Updated: Jun 12, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Risk of bias assessment tool for systematic review and meta-analysis of the gut microbiome
Thomas Lampeter1, Charles Love2, Trien T Tang2
1New York Institute of Technology College of Osteopathic Medicine, Glen Head, NY, USA.
Abstract:
Risk of bias assessment is a critical step of any meta-analysis or systematic review. Given the low sample count of many microbiome studies, especially observational or cohort studies involving human subjects, many microbiome studies have low power. This increases the importance of performing meta-analysis and systematic review for microbiome research in order to enhance the relevance and applicability of microbiome results. This work proposes a method based on the ROBINS-I tool to systematically consider sources of bias in microbiome research seeking to perform meta-analysis or systematic review for microbiome studies.
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