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Related Concept Videos

Cystic Fibrosis: Management01:24

Cystic Fibrosis: Management

145
Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...
145
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

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Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
195

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Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
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Modeling Cystic Fibrosis Chronic Infection Using Engineered Mucus-like Hydrogels.

Courtney L O'Brien1, Sarah Spencer1, Naeimeh Jafari2

  • 1School of Biomedical Engineering, Faculties of Medicine and Engineering, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada.

ACS Biomaterials Science & Engineering
|September 19, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed mucus-like hydrogels to model cystic fibrosis (CF) airway infections. These models revealed that antibiotic resistance in CF airways depends on mucus properties, microbial interactions, and host cells.

Keywords:
biomaterialschronic infectioncoculturecystic fibrosisdisease modelingmicrobiology

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Area of Science:

  • Biomedical Engineering
  • Microbiology
  • Pulmonary Medicine

Background:

  • Cystic fibrosis (CF) airway mucus has altered properties, promoting difficult-to-treat chronic infections.
  • Modeling complex polymicrobial infections and host-microbe interactions in vitro is challenging.

Purpose of the Study:

  • To develop mucus-like hydrogels that mimic healthy and CF airway environments.
  • To investigate factors influencing pathogen antibiotic resistance in these models.

Main Methods:

  • Created mucus-like hydrogels with varying compositions and viscoelastic properties.
  • Incorporated hydrogels with pathogens, human bronchial epithelial cells, and antibiotics.
  • Assessed pathogen antibiotic resistance under different culture conditions.

Main Results:

  • Hydrogel properties influenced, but did not solely determine, antibiotic resistance.
  • Culture conditions (microbe species, polymicrobial vs. monomicrobial, presence of epithelial cells) significantly impacted resistance.
  • The CF airway model replicated sustained polymicrobial growth and increased antibiotic tolerance.

Conclusions:

  • Developed a novel in vitro model for studying CF airway infections.
  • Demonstrated that antibiotic resistance is multifactorial, involving mucus properties, microbial dynamics, and host cell interactions.
  • The model provides insights into the challenges of treating chronic CF lung infections.