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Updated: Jun 12, 2025

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
Published on: May 12, 2023
Biochemical and biophysical approaches to characterization of the aromatic amino acid hydroxylases
Paul F Fitzpatrick1, S Colette Daubner1
1Department of Biochemistry and Structural Biology, UT Health San Antonio, San Antonio, TX, United States.
Abstract:
The aromatic amino acid hydroxylases phenylalanine hydroxylase, tyrosine hydroxylase, and tryptophan hydroxylase utilize a non-heme iron to catalyze the hydroxylation of the aromatic rings of their amino acid substrates, with a tetrahydropterin serving as the source of the electrons necessary for the monooxygenation reaction. These enzymes have been subjected to a variety of biochemical and biophysical approaches, resulting in a detailed understanding of their structures and mechanism. We summarize here the experimental approaches that have led to this understanding.
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