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Updated: Jul 18, 2026

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation
Published on: April 27, 2019
Surgical techniques for cardiac procurement, preparation and perfusion using the Organ Care System
Manuel Egle1, Sarah Longnus2, Murat Yildiz3
1Department of Cardiac Surgery, Inselspital, Bern University Hospital, Bern, Switzerland Department for BioMedical Research, University of Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland.
Abstract:
We provide an audio-visual step-by-step guide to the preparation of a donor heart for the application of normothermic, ex situ cardiac perfusion on the TransMedics Organ Care System using a heart donated after brain death. The use of the Organ Care System increases heart transplantation activity by enabling the utilization of hearts donated after circulatory death, the use of extended criteria grafts and the extension of out-of-body time, which can help overcome geographic or surgical barriers. Ex situ cardiac perfusion is a new technique and is therefore not yet routinely performed in many centres. However, it can be assumed that this technique will become more established and widespread in the future. Our video tutorial, which summarizes all important steps, can therefore be of benefit to surgical teams for planning, training or as a refresher.
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