Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

205.0K
Overview
205.0K
DNA Isolation01:24

DNA Isolation

38.4K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
38.4K
Viral Recombination00:57

Viral Recombination

23.3K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.3K
Real Time RT-PCR02:57

Real Time RT-PCR

56.9K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.

Analytical chemistry·2026
Same author

Validation of the REverSe TRanscrIptase Chain Termination assay for measuring tenofovir diphosphate in dried blood spots from a clinical pharmacokinetic trial.

The Journal of antimicrobial chemotherapy·2025
Same author

SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control.

Sensors & diagnostics·2024
Same author

REverSe TRanscrIptase chain termination (RESTRICT) for selective measurement of nucleotide analogs used in HIV care and prevention.

Bioengineering & translational medicine·2023
Same author

Rapid detection of hepatitis C virus using recombinase polymerase amplification.

PloS one·2022
Same author

Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis.

Lab on a chip·2022

Related Experiment Video

Updated: Jun 12, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.6K

Vibration mixing for enhanced paper-based recombinase polymerase amplification.

Kelli N Shimazu1, Andrew T Bender1, Per G Reinhall1

  • 1Department of Mechanical Engineering, University of Washington, Stevens Way, Box 352600, Seattle, Washington, 98195, USA. jposner@uw.edu.

Lab on a Chip
|September 20, 2024
PubMed
Summary

This study introduces a vibration mixing platform to enhance isothermal nucleic acid amplification tests (NAATs) on paper. This innovation significantly improves detection limits and speed for point-of-care diagnostics.

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

173
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K

Related Experiment Videos

Last Updated: Jun 12, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.6K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

173
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K

Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Molecular Biology

Background:

  • Isothermal nucleic acid amplification tests (NAATs) are crucial for point-of-care (POC) diagnostics, offering advantages over traditional PCR.
  • Paper-based diagnostic devices are promising for affordable POC NAATs but face challenges with reagent integration and low detection limits.
  • Recombinase polymerase amplification (RPA), a rapid isothermal NAAT, struggles in porous membranes due to viscous reaction requirements.

Purpose of the Study:

  • To improve the performance of membrane-based recombinase polymerase amplification (RPA) for detecting HIV-1 DNA and viral RNA.
  • To overcome limitations of paper-based NAATs by developing an active mixing strategy.
  • To enable rapid, sensitive, and low-cost POC diagnostics.

Main Methods:

  • Development and implementation of a coin cell-based vibration mixing platform for membrane-based RPA.
  • Utilizing a low-cost vibration motor for simultaneous temperature control and mixing.
  • Assessing the limit of detection, time to threshold, and fluorescence output of the mixed reactions.

Main Results:

  • Achieved a limit of detection of 12 copies of DNA per reaction.
  • Reduced the time to threshold by approximately 50% (from ~10 minutes to ~5 minutes).
  • Increased overall fluorescence output by up to 16-fold compared to unmixed experiments.

Conclusions:

  • A vibration mixing platform significantly enhances membrane-based RPA performance for POC diagnostics.
  • This active mixing strategy allows for effective amplification even when target and reaction components are initially separated.
  • The developed system offers a low-cost, efficient solution for sensitive nucleic acid detection at the point of care.