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Establishment and characterization of a novel human papillomavirus-positive tonsillar squamous cell carcinoma cell line (KUSCC-152) derived from a Korean patient

  • 0Department of Otorhinolaryngology - Head and Neck Surgery, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul, South Korea.
Acta Oto-Laryngologica +

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September 20, 2024

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September 20, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

A new human papillomavirus (HPV)-positive tonsillar squamous cell carcinoma (TSCC) cell line, KUSCC-152, was established from an East Asian patient. This integrated-type cell line exhibits cancer stemness and tumorigenicity, aiding TSCC research.

Area Of Science

  • Oncology
  • Virology
  • Cell Biology

Background

  • Human papillomavirus (HPV) is implicated in tonsillar squamous cell carcinoma (TSCC).
  • Limited availability of authenticated HPV-positive TSCC cell lines hinders research.
  • Understanding HPV-induced TSCC mechanisms requires robust cellular models.

Purpose Of The Study

  • To establish and characterize a novel, <i>de novo</i> HPV-positive TSCC cell line from a Korean patient.
  • To evaluate the tumorigenicity and stemness properties of the new cell line.
  • To determine the HPV integration status within the TSCC cell line.

Main Methods

  • <i>In vitro</i> and <i>in vivo</i> experiments were conducted to assess tumorigenicity.
  • Cancer stemness phenotype was evaluated through sphere-forming capacity and marker expression.
  • Physical status of HPV (episomal, integrated, or mixed) was examined.

Main Results

  • A novel TSCC cell line, KUSCC-152, was established and confirmed to be HPV-16 positive.
  • KUSCC-152 cells demonstrated <i>in vitro</i> cancer cell traits and induced tumor formation and metastasis in xenograft mice.
  • The cell line exhibited cancer stemness characteristics, including sphere formation and stemness marker expression.
  • HPV in KUSCC-152 cells was suggested to be of the integrated type.

Conclusions

  • A novel, integrated-type HPV-positive TSCC cell line (KUSCC-152) was successfully established and characterized.
  • This cell line, derived from an East Asian patient, provides a valuable tool for studying HPV-induced TSCC.
  • The findings contribute to a better understanding of HPV-driven oncogenesis in the tonsil.

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