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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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nQuant Enables Precise Quantitative N-Glycomics.

Yudong Guan1, Shanshan Zhao2, Chunjin Fu3

  • 1Department of Critical Care Medicine, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong 518020, China.

Analytical Chemistry
|September 20, 2024
PubMed
Summary
This summary is machine-generated.

We developed nQuant, a new glycoinformatics tool for precise N-glycan quantification. This tool supports both label-free and isotopic labeling methods, improving the analysis of N-glycomics data from mass spectrometry.

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Area of Science:

  • Biochemistry
  • Glycomics
  • Bioinformatics

Background:

  • N-glycosylation is a critical post-translational modification influencing protein function.
  • Aberrant N-glycosylation is implicated in numerous human diseases.
  • Current glycoinformatics tools lack the precision required for comprehensive N-glycan quantification.

Purpose of the Study:

  • To develop a novel glycoinformatics tool, nQuant, for accurate and efficient quantification of N-glycans.
  • To enable both label-free and isotopic labeling quantification of N-glycomics data.
  • To address the limitations of existing tools in high-precision N-glycan analysis.

Main Methods:

  • Development of the nQuant software tool for N-glycomics data analysis.
  • Utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for data acquisition.
  • Implementation of label-free and isotopic labeling quantification modules within nQuant.

Main Results:

  • nQuant demonstrated a low false quantitation rate for N-glycans.
  • Profiled N-glycans from purified glycoproteins, HEK293 cells, and during mouse corpus callosum development using label-free quantification.
  • Revealed dynamic N-glycan changes in acute promyelocytic leukemia cells post-treatment using isotopic labeling quantification.

Conclusions:

  • nQuant provides a fast and precise method for quantitative N-glycomics.
  • The tool facilitates the study of N-glycosylation dynamics in various biological contexts.
  • nQuant enhances the capability to analyze complex glycomics data for disease research.