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An amygdalar oscillator coordinates cellular and behavioral rhythms.

Qiang Liu1, Jiali Xiong2, Dong Won Kim3

  • 1Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

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Summary

Researchers identified a novel brain oscillator in the lateral amygdala (LA) that regulates circadian rhythms. This oscillator, defined by mWAKE expression, controls behavioral rhythms and sensory perception.

Keywords:
SCNanxietycircadianclocklateral amygdalalocal clockmWAKErhythmtouch

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Area of Science:

  • Neuroscience
  • Chronobiology
  • Molecular Biology

Background:

  • Circadian rhythms are regulated by the suprachiasmatic nucleus (SCN) and local body clocks.
  • The specific brain regions outside the SCN that generate rhythmic behaviors are not fully understood.

Purpose of the Study:

  • To identify and characterize extra-SCN brain oscillators responsible for rhythmic behaviors.
  • To elucidate the molecular mechanisms underlying these oscillators.

Main Methods:

  • Utilized molecular markers like mWAKE/ANKFN1 to identify neuronal populations.
  • Performed genetic manipulation to disrupt clock function and excitatory signaling in specific neuronal subsets.
  • Measured Period2 (PER2) rhythms and neuronal excitability.
  • Assessed behavioral outputs related to sensory perception and anxiety.

Main Results:

  • Identified mWAKE-expressing neurons in the anterior/dorsal lateral amygdala (adLA) as a key extra-SCN oscillator.
  • Disruption of clock function or signaling in adLAmWAKE neurons eliminated PER2 rhythms in the LA.
  • mWAKE levels exhibit nocturnal rise, enhancing adLAmWAKE neuronal excitability via Ca2+-activated K+ channels.
  • adLAmWAKE neurons coordinate rhythmic sensory perception and anxiety in a WAKE-dependent manner.

Conclusions:

  • Revealed the cellular identity of an extra-SCN brain oscillator in the lateral amygdala.
  • Proposed a hierarchical system for organizing molecular and behavioral rhythms, involving the SCN and extra-SCN oscillators.