Exploring novel potential of mycosynthesized magnetic nanoparticles for phosphatase immobilization and biological...

Among different microbes, fungi are proficient candidates for the extracellular synthesis of iron nanoparticles. For biogenic synthesis of iron nanoparticles, a thermophilic mould Myceliophthora thermophila BJTLRMDU7 was used in this study. Mycogenic magnetic nanoparticles were used for phosphatase immobilization and therapeutic applications such as antimicrobial and antimalarial activity. Firstly, the phosphatase was immobilized on biogenic iron nanoparticles with an efficiency of >56 %. Immobilized enzyme was optimally active at 60 °C and pH 5. Immobilized phosphatase was recycled using external magnetic field up to 4th cycle retaining >50 % activity. The immobilized phosphatase efficiently released inorganic phosphate from different flours such as wheat, maize and gram at 37 °C and 60 °C. There was continuous increase in the release of inorganic phosphorus from all samples with incubation time at 37 °C and slight reduction at 60 °C. These nanoparticles showed the effective antimicrobial activity against Bacillus subtilis, Escherichia coli and Myceliophthora thermophila. Further, the synthesized iron nanoparticles showed antimalarial potential against Plasmodium falciparum. Biogenic nanoparticles did not exhibit hemolytic activity and cytotoxicity. Therefore, biogenic iron nanoparticles could be used as a suitable matrix for immobilization of enzymes and safe therapeutics.