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Updated: Jun 12, 2025

Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

Engineering extracellular vesicles for diagnosis and therapy.

Zhengyue Fei¹, Jiamin Zheng¹, Xiangxiang Zheng²

¹College of Biological and Pharmaceutical Engineering, Nanjing Tech University, Jiangsu Province, China.

Trends in Pharmacological Sciences

|September 20, 2024

Summary

This summary is machine-generated.

Bio-orthogonal click chemistry enables precise engineering of extracellular vesicles (EVs) for improved therapeutic applications. This approach enhances EV targeting and function, overcoming limitations in clinical translation for drug delivery and regenerative medicine.

Keywords:

bio-orthogonal click chemistry bioimaging diagnosis extracellular vesicles isolation labeling membrane insertion metabolic engineering targeted therapy

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Last Updated: Jun 12, 2025

Extraction of Extracellular Vesicles from Whole Tissue

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Published on: February 7, 2019

Characterizing Extracellular Vesicles from Biological Fluids

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Published on: February 28, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

Biotechnology
Nanomedicine
Molecular Engineering

Background:

Extracellular vesicles (EVs) show promise in drug delivery, immunotherapy, and regenerative medicine.
Clinical translation of EVs is hindered by low yields, functional variability, and poor targeting.
Engineering EVs can enhance their therapeutic efficacy and overcome current limitations.

Purpose of the Study:

To provide an overview of bio-orthogonal labeling strategies for extracellular vesicle (EV) engineering.
To discuss methods for isolating bio-orthogonally labeled EVs.
To highlight in vivo applications of engineered EVs in diagnosis and therapy.

Main Methods:

Utilizing bio-orthogonal click chemistry for controlled and specific modification of EVs.
Employing magnetic beads, microfluidics, and microarray chips for isolation of labeled EVs.
Investigating glycometabolic engineering of EVs for targeted therapeutic delivery.

Main Results:

Bio-orthogonal click chemistry allows for precise engineering of EVs without disrupting their structure.
Effective isolation techniques for bio-orthogonally labeled EVs have been presented.
Engineered EVs demonstrate potential for in vivo diagnosis and targeted therapies.

Conclusions:

Bio-orthogonal labeling is a powerful tool for advancing extracellular vesicle (EV) engineering.
Engineered EVs offer improved targeting and functionality for clinical applications.
Glycometabolic engineered EVs hold significant potential for future targeted therapies.