Development of Highly Sensitive Immunochromatography Using Time-Resolved Fluorescence Microspheres for Amantadine...

Area of Science:

• Food Safety
• Veterinary Pharmacology
• Analytical Chemistry

Background:

• Amantadine (AMA) is a banned drug in livestock due to human health risks.
• Unauthorized AMA use in food animals poses a significant public health concern.
• Effective detection methods are crucial for monitoring and enforcing AMA bans.

Purpose of the Study:

• To develop a sensitive and specific detection method for unauthorized Amantadine (AMA) in livestock.
• To create a reliable screening tool for illegal AMA usage in chicken muscle.
• To ensure food safety by preventing the consumption of meat contaminated with AMA.

Main Methods:

• Rational design of a polyclonal antibody targeting Amantadine (AMA).
• Computational chemistry analysis to validate hapten design.
• Establishment of a time-resolved fluorescence immunochromatographic assay (TRFICA) using the developed antibody.
• Validation of the TRFICA method with recovery rates and comparison against HPLC-MS/MS.

Main Results:

• A high-affinity polyclonal antibody for Amantadine (AMA) was successfully developed.
• The established TRFICA demonstrated a visual detection limit of 0.6 μg/kg and a quantitative limit of 0.05 μg/kg.
• The assay exhibited excellent recovery rates (94.5–109.9%) and low variability (≤10%), aligning with HPLC-MS/MS results.

Conclusions:

• The developed TRFICA is a highly responsive and accurate tool for detecting illegal Amantadine (AMA) in chicken muscle.
• This assay serves as an ideal screening and monitoring solution for enforcing AMA bans in the livestock industry.
• The findings contribute to enhancing food safety and protecting human well-being from drug residues in meat products.