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Related Experiment Video

Updated: Jun 12, 2025

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Qishen Granules Modulate Metabolism Flexibility Against Myocardial Infarction via HIF-1 α-Dependent Mechanisms in

Xiao-Qian Sun1,2, Xuan Li1,2, Yan-Qin Li1,2

  • 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.

Chinese Journal of Integrative Medicine
|September 21, 2024
PubMed
Summary

Qishen Granules (QSG) improve heart failure by regulating myocardial energy metabolism via the HIF-1α pathway. This enhances cardiac function in ischemic heart failure rats.

Keywords:
Qishen Granulesenergy metabolismheart failurehypoxia-inducible factor-1 α pathwaysubstrate utilization

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Area of Science:

  • Cardiology
  • Metabolic Research
  • Pharmacology

Background:

  • Heart failure (HF) is a complex condition characterized by impaired cardiac function.
  • Myocardial energy metabolism disorders are central to ischemic injury and HF progression.
  • Targeting metabolic pathways offers a potential therapeutic strategy for HF.

Purpose of the Study:

  • To evaluate the cardioprotective effects of Qishen Granules (QSG) in a rat model of heart failure.
  • To investigate how QSG modulates glucose and fatty acid metabolism in ischemic myocardium.
  • To elucidate the underlying molecular mechanisms, particularly the role of the HIF-1α pathway.

Main Methods:

  • Established a rat model of heart failure via left anterior descending ligation.
  • Assessed cardiac function using echocardiography and histology.
  • Utilized positron emission tomography (PET) for glucose metabolism imaging.
  • Analyzed fatty acid metabolism and ATP production through biochemical assays.
  • Investigated molecular mechanisms using a hypoxia-induced H9C2 cell model, flow cytometry, and immunofluorescence for HIF-1α.

Main Results:

  • QSG treatment improved cardiac function in heart failure rats.
  • QSG modulated glucose and fatty acid metabolism patterns in ischemic and remote myocardial areas.
  • QSG promoted glucose metabolism under high hypoxia and lipid metabolism under low hypoxia, mediated by HIF-1α.
  • QSG regulated HIF-1α nuclear translocation, influencing compensatory metabolic adjustments.

Conclusions:

  • QSG exerts cardioprotective effects by rebalancing myocardial energy metabolism in ischemic regions.
  • The mechanism involves regulating HIF-1α protein stability to coordinate substrate utilization.
  • QSG alleviates energy metabolism disorders, offering a therapeutic approach for ischemic heart injury.