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Related Experiment Video

Updated: Jun 12, 2025

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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VrNIN1 interacts with VrNNC1 to regulate root nodulation in mungbean.

Yanzheng Zhang1, Rui Hou1, Xiaolin Yao1

  • 1College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.

Plant Physiology and Biochemistry : PPB
|September 21, 2024
PubMed
Summary

The VrNIN1 gene regulates root nodule symbiosis in mungbean. Its manipulation affects nodule formation, interacting with VrNNC1 to control this crucial legume process.

Keywords:
Hairy rootNodulationVigna radiataVrNIN1VrNNC1

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Area of Science:

  • Plant Molecular Biology
  • Legume Symbiosis Research
  • Genetics and Genomics

Background:

  • Node Inception (NIN) is vital for legume symbiosis, but its role in mungbean (Vigna radiata) is unclear.
  • Understanding NIN's function is key to improving nitrogen fixation in legumes.

Purpose of the Study:

  • To functionally characterize the VrNIN1 gene in mungbean.
  • To elucidate the regulatory mechanisms of root nodule symbiosis involving VrNIN1.

Main Methods:

  • Enhanced hairy root transformation and GUS staining for gene expression analysis.
  • Yeast two-hybrid, luciferase complementation, and Co-immunoprecipitation assays for protein interactions.
  • Gene expression analysis of downstream regulators like VrRIC1 and VrEDOD40.

Main Results:

  • VrNIN1 is expressed in root primordia and nodules, and its altered expression significantly impacts nodule number.
  • VrNIN1 interacts with VrNNC1, which suppresses nodule formation.
  • Both VrNIN1 and VrNNC1 regulate nodulation by modulating VrRIC1 and VrEDOD40 expression.

Conclusions:

  • VrNIN1 plays a critical role in mungbean root nodule symbiosis.
  • The study provides mechanistic insights into VrNIN1 and VrNNC1 regulatory functions.
  • An optimized hairy root system for mungbean was established.