Determination of dextranase activity using 3-methyl-2-benzothiazolinone hydrazone method: Substrate refinement and
Jingwen Dong1, Yinchun Liu1, Fang Liu1
1State Key Laboratory Base of Eco-chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, No.53 Zhengzhou Road, Qingdao 266042, China.
Food Chemistry
|September 21, 2024
Summary
A new, highly sensitive assay accurately measures dextranase activity using 3-methyl-2-benzothiazolinone hydrazine. This optimized method is reliable for analyzing enzymatic hydrolysis in products like toothpaste.
Area of Science:
- Enzymology
- Analytical Chemistry
Background:
- Dextranase activity measurement is crucial for various applications.
- Existing methods, such as the 3,5-dinitrosalicylic acid method, face limitations like interference and lower sensitivity.
Purpose of the Study:
- To develop and validate a highly sensitive and accurate method for measuring dextranase activity.
- To establish a robust assay applicable to complex matrices like toothpaste and mouthwash.
Main Methods:
- Development of a novel assay using 3-methyl-2-benzothiazolinone hydrazine.
- Optimization of measurement parameters including standard curve analysis (slope, intercept, R²).
- Kinetic determination for optimizing enzymatic parameters and validating the assay.
Main Results:
- The method demonstrates high sensitivity, accuracy, and repeatability within the 0.7-6.5 mU/mL range.
- Enzymatic hydrolysis follows a zero-order reaction, suitable for end-point assays.
- Successful application in analyzing dextranase activity in toothpaste and mouthwash, outperforming existing methods.
Conclusions:
- The developed 3-methyl-2-benzothiazolinone hydrazine method provides a superior alternative for dextranase activity quantification.
- This assay overcomes interference issues present in complex samples, offering reliable results.
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