A smartphone-based multichannel magnetoelastic immunosensor for acute aortic dissection supplementary diagnosis

Area of Science:

• Biomedical Engineering
• Biosensing Technology
• Medical Diagnostics

Background:

• Acute aortic dissection (AAD) diagnosis relies on biomarkers such as C-reactive protein (CRP), smooth muscle myosin heavy chain (SmMHC), and D-dimer (D-D).
• Current diagnostic methods require sophisticated instruments, complex procedures, and expensive reagents, limiting accessibility for real-time monitoring and home use.

Purpose of the Study:

• To develop a smartphone-based multichannel magnetoelastic (ME) immunosensing device for accessible, real-time detection of AAD protein biomarkers.
• To overcome the limitations of current diagnostic methods by offering a simpler, cost-effective solution for chronic disease monitoring and home self-examination.

Main Methods:

• Development of a smartphone-integrated multichannel ME immunosensor for detecting protein levels.
• Measurement of resonance frequency shift (RFS) in response to antibody-antigen binding for target detection.
• Quantitative analysis of CRP, SmMHC, and D-D concentrations within specific ranges.

Main Results:

• The ME immunosensor demonstrated a linear relationship between biomarker concentration (CRP, SmMHC, D-D) and RFS within specified ranges.
• Achieved high sensitivities for CRP (13.37Hz/μg∙mL⁻¹), SmMHC (155.19Hz/ng∙mL⁻¹), and D-D (332.72Hz/μg∙mL⁻¹).
• Detection limits were as low as 2.634×10⁻³μg/mL for CRP, 1.155×10⁻²ng/mL for SmMHC, and 3.687×10⁻³μg/mL for D-D, with accuracy and stability comparable to VNA.

Conclusions:

• The developed smartphone-based ME immunosensing device provides an effective and accessible tool for supplementary AAD diagnosis.
• This technology facilitates real-time monitoring and home self-examinations, improving patient care for potential AAD cases.
• The device's performance indicates its potential to replace complex laboratory-based diagnostic methods for certain biomarkers.