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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: May 9, 2026

Indocyanine Green-Guided Intraoperative Imaging to Facilitate Video-Assisted Retroperitoneal Debridement for Treating Acute Necrotizing Pancreatitis

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Indocyanine Green (ICG): A Versatile Tool in Enhancing Precision in Minimally Invasive Thoracic Surgery.

Oscar Zhang¹, Gavin Wright², Yin-Kai Chao³

¹Department of Cardiothoracic Surgery, Royal Prince Alfred Hospital, The University of Sydney, Sydney, NSW, Australia; Chris O'Brien Lifehouse, Sydney, NSW, Australia.

Heart, Lung & Circulation

|September 21, 2024

Summary

Indocyanine green (ICG) fluorescence imaging enhances precision in minimally invasive thoracic surgery. This technique aids in identifying critical anatomical structures and guiding safe surgical dissection.

Keywords:

Indocyanine green Near-infrared (NIR) fluorescence imaging Robotic surgery

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Area of Science:

Thoracic Surgery
Surgical Imaging
Minimally Invasive Procedures

Background:

Intraoperative fluorescence imaging offers real-time visualization during surgery.
Minimally invasive thoracic surgery benefits from enhanced visualization tools.
Indocyanine green (ICG) is a near-infrared fluorescent agent used in various medical applications.

Observation:

ICG fluorescence imaging was utilized in minimally invasive thoracic procedures.
The study explored multiple clinical applications of ICG, including identifying intersegmental planes, locating thoracic duct injuries, and mapping sentinel lymph nodes.
Real-time visualization of ICG allowed for clear identification of intra-thoracic structures.

Findings:

ICG fluorescence imaging successfully identified key anatomical structures and boundaries within the thorax.
Specific applications demonstrated include identifying intersegmental planes for precise resection.
The technique was effective in locating thoracic duct injuries and anomalous systemic arteries in pulmonary sequestration.

Implications:

ICG fluorescence imaging improves surgical precision and safety in thoracic procedures.
This technology facilitates accurate dissection by clearly delineating anatomical targets.
The findings support the broader adoption of ICG for enhanced intraoperative guidance in thoracic surgery.