Related Experiment Video
Updated: Jun 12, 2025
Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
Published on: April 23, 2021
Protocol for formation, staining, and imaging of 3D breast cancer models using MicroTissues mold systems
Ben Haspels1, Maayke M P Kuijten1
1Department of Molecular Genetics, Erasmus MC, 3000 CA, Rotterdam, the Netherlands; Oncode Institute, Erasmus MC Cancer Institute, Erasmus MC, 3015 GD, Rotterdam, the Netherlands.
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The use of 3D cancer models to study therapy response has a great advantage over conventional 2D cell culture. Here, we present an optimized protocol for breast cancer spheroid and rod-shaped microtissue formation using MicroTissues mold systems. We describe steps for cast formation and treating the 3D models with DNA-damaging agents. We then detail procedures for analyzing the 3D models by whole-mount immunostaining and confocal imaging of fixed samples or with the use of live-cell reporters.
