Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caffeine suppresses inflammation and subretinal fibrosis through modulation of the thrombospondin-1-Bim axis.

Experimental eye research·2026
Same author

Emerging Roles of Regulated Cell Death-mediated Inflammation in Pathophysiology of Ocular Diseases.

Journal of ophthalmic & vision research·2026
Same author

Components and Delivery Formats of Cognitive Behavioral Therapy for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis: A Systematic Review and Component Network Meta-Analysis.

Brain and behavior·2026
Same author

Suboptimal Responses to Anti-VEGF in Retinal Neurovascular Diseases: Linking Aging and Alternative Angioinflammatory Pathways.

Investigative ophthalmology & visual science·2026
Same author

Diabetes associated pericyte metabolic signatures and pathogenesis of diabetic retinopathy.

Frontiers in endocrinology·2026
Same author

Caffeine Mitigates Adenosine-Mediated Angiogenic Properties of Choroidal Endothelial Cells Through Antagonism of A<sub>1</sub> Adenosine Receptor and PI3K-AKT Axis.

Cells·2026
Same journal

Corneal deformation mapping and FE-based strain analysis via digital image correlation: Biomechanical changes after CXL and laser refractive surgery.

Experimental eye research·2026
Same journal

Tanshinone IIA inhibits choroidal neovascularization and restores outer blood-retinal barrier function in Vldlr knockout mice.

Experimental eye research·2026
Same journal

Understanding the ocular accumulation of mefuparib and its N-dealkylation metabolite: Pharmacokinetics, melanin affinity, and cellular disposition.

Experimental eye research·2026
Same journal

Mitochondrial Dysfunction and Diabetic Retinopathy: Research Progress from Pathogenic Mechanisms to Therapeutic Targets.

Experimental eye research·2026
Same journal

Middle-wavelength green ambient light attenuates lens-induced myopia progression and is associated with suppression of the Wnt/β-catenin signaling pathway in guinea pigs.

Experimental eye research·2026
Same journal

Experimental Corneal Alkali Burn Models: Methodological Standards, Biological Outcomes, and Translational Gaps.

Experimental eye research·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

3.4K

Bax expression impacts postnatal retinal vascular development and hyperoxia sensitivity.

Nader Sheibani1, Yanzhi Sang2, Shoujian Wang2

  • 1Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA; McPherson Eye Research Institute, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA; Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.

Experimental Eye Research
|September 22, 2024
PubMed
Summary
This summary is machine-generated.

The Bim-Bax pathway removes excess retinal endothelial cells but not pericytes during development and oxygen-induced ischemic retinopathy. Additional Bim pathways are needed for pericyte removal.

More Related Videos

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

8.4K
Author Spotlight: Understanding Retinal Vessel Resilience and Disease Progression
04:36

Author Spotlight: Understanding Retinal Vessel Resilience and Disease Progression

Published on: January 12, 2024

1.0K

Related Experiment Videos

Last Updated: Jun 12, 2025

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

3.4K
Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

8.4K
Author Spotlight: Understanding Retinal Vessel Resilience and Disease Progression
04:36

Author Spotlight: Understanding Retinal Vessel Resilience and Disease Progression

Published on: January 12, 2024

1.0K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Developmental Biology

Background:

  • Apoptosis is crucial for organ development and homeostasis.
  • Bcl-2 family proteins regulate apoptosis via the intrinsic cell death pathway.
  • Bim activates Bax/Bak to induce apoptosis, impacting retinal vascularization and oxygen-induced ischemic retinopathy (OIR).

Purpose of the Study:

  • To investigate the role of Bax in retinal vascular development and OIR.
  • To determine if the Bim-Bax pathway is sufficient for endothelial cell and pericyte removal.
  • To elucidate the necessity of additional Bim-mediated pathways for pericyte homeostasis.

Main Methods:

  • Comparative analysis of retinal vascularization in Bax knockout (Bax-/-) mice versus wild-type (Bax+/+) mice.
  • Assessment of hyperoxia-mediated vessel obliteration in Bax-/- mice during OIR.
  • Comparison of vascular phenotypes in Bax-/- and Bim-/- mice.

Main Results:

  • Bax-/- retinas exhibited increased endothelial cell and pericyte numbers compared to Bax+/+ mice.
  • Absence of Bax significantly reduced hyperoxia-mediated vessel obliteration during OIR.
  • While endothelial cell increases in Bax-/- mice were similar to Bim-/- mice, pericyte increases were less pronounced, indicating partial protection.

Conclusions:

  • The Bim-Bax pathway effectively removes excess retinal endothelial cells but not pericytes during postnatal development and OIR.
  • Additional Bim-mediated pathways are essential for pericyte removal and mitigating hyperoxia-induced vascular damage.
  • This highlights distinct roles for Bim in regulating endothelial cells versus pericytes.