Nucleic Acid Amplification Circuit-Based Hydrogel (NACH) Assay for One-Step Detection of Metastatic Gastric Cancer-Derived Exosomal miRNA

Seung Beom Seo ^1,2, Jaewoo Lim ^1,3, Kyujung Kim ²

¹Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
²Department of Cogno-Mechatronics Engineering, Pusan National University, Pusan, 46241, Republic of Korea.
³Medical Device Development Center, Osong Medical Innovation Foundation, 123, Osongsaengmyeong-ro, Chungcheongbuk-do, 28160, Republic of Korea.
⁴YUHS-KRIBB Medical Convergence Research Institute, Yonsei University, Seoul, 03772, Republic of Korea.
⁵Department of Radiology, Yonsei University, Seoul, 03772, Republic of Korea.
⁶Department of Bioengineering & Nano-bioengineering, Research Center for Bio Materials and Process Development, Incheon National University, Incheon, 22012, Republic of Korea.
⁷Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
⁸MediBio-Informatics Research Center, Novomics Co., Ltd., Seoul, 07217, Republic of Korea.
⁹School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
¹⁰Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, Daejeon, 34113, Republic of Korea.
¹¹Department of Biochemistry & Molecular Biology, College of Medicine, Yonsei University, Seoul, 03722, Republic of Korea.

⁰Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.

Advanced Science (weinheim, Baden-Wurttemberg, Germany) +

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September 23, 2024

View abstract on PubMed

Summary

This study introduces a novel nucleic acid amplification circuit-based hydrogel (NACH) assay for detecting exosomal microRNAs in metastatic gastric cancer (GC). The assay offers rapid, sensitive, on-site detection for improved GC diagnostics.

Area Of Science

Biochemistry
Molecular Biology
Oncology

Background

Gastric cancer (GC) is a prevalent malignancy with diverse prognoses.
Epithelial-mesenchymal transition (EMT) promotes GC invasion and metastasis.
Exosomal microRNAs (miRNAs) are key biomarkers in cancer progression.

Purpose Of The Study

To develop a nucleic acid amplification circuit-based hydrogel (NACH) assay for identifying exosomal miRNAs in metastatic GC.
To enable rapid, sensitive, and on-site detection of specific cancer-related miRNAs.

Main Methods

Utilized rolling circle amplification within a hydrogel matrix.
Targeted specific miRNAs: miRNA-21 (oncogene) and miRNA-99a (EMT promoter).
Immobilized specific amplification probes for a one-step reaction.

Main Results

Achieved a detection limit of 1 fM for both miRNA-21 and miRNA-99a.
Demonstrated comparable fluorescence intensity to qRT-PCR using various sample types.
Validated diagnostic performance with clinical GC patient samples using a portable fluorometer.

Conclusions

The NACH assay provides a robust platform for sensitive, on-site detection of exosomal miRNAs.
This technology holds significant potential for the genetic diagnosis and point-of-care testing of gastric cancer.

Keywords:

exosomal miRNA gastric cancer hydrogel assay nucleic acid amplification circuit portable fluorometer