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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 12, 2025

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
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Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

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Agreement of lymphocyte subsets detection permits reference intervals transference between flow cytometry systems:

Mei Liu1, Sihua Yu1,2,3, Siyao Li1

  • 1National Clinical Research Center for Laboratory Medicine, The First Hospital of China Medical University, Shenyang, China.

Clinical Chemistry and Laboratory Medicine
|September 23, 2024
PubMed
Summary

Reference intervals for lymphocyte subsets are transferable between different flow cytometry systems, simplifying clinical laboratory diagnostics. This study demonstrates successful transfer across multiple platforms, excluding NK cells, for improved accuracy.

Keywords:
flow cytometrylymphocyte subsetsreference intervalstransference

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Area of Science:

  • Clinical Immunology
  • Flow Cytometry Technology
  • Laboratory Medicine

Background:

  • Flow cytometry (FCM) is crucial for lymphocyte subset detection in clinical settings.
  • The proliferation of single-platform FCM systems necessitates standardized reference intervals (RIs).
  • Transferring RIs between FCM systems offers a practical solution for laboratories.

Purpose of the Study:

  • To investigate the transferability of lymphocyte subset reference intervals (RIs) across different single-platform flow cytometry systems.
  • To evaluate the feasibility of establishing and transferring RIs for lymphocyte subsets.
  • To compare RIs across NovoCyte, BriCyteE6, DxFLEX, and FACSCantoII platforms.

Main Methods:

  • Performed pairwise method comparisons across four FCM platforms: NovoCyte, BriCyteE6, DxFLEX, and FACSCantoII.
  • Evaluated the transferability of established lymphocyte subset RIs.
  • Transferred RIs from FACSCantoII to BriCyteE6 and DxFLEX systems (excluding NK cells) and verified them by calculating bias (CV).

Main Results:

  • Lymphocyte subset detection results were comparable across the four evaluated FCM systems.
  • The transfer of RIs for lymphocyte subsets between the tested FCM systems was feasible.
  • Transferred RIs showed a coefficient of variation (CV) below 20% compared to established RIs for most parameters, excluding NK cells.

Conclusions:

  • Reference intervals for lymphocyte subsets can be successfully transferred between different flow cytometry systems.
  • Transferability is demonstrated across NovoCyte, BriCyteE6, DxFLEX, and FACSCantoII platforms.
  • NK cells represent an exception, with RIs not being transferable due to different definition strategies.