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Related Concept Videos

The Tumor Microenvironment02:17

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Single cell RNA-Sequencing Reveals Mast Cells Enhance Mononuclear Phagocytes Infiltration in Bladder Cancer

Zige Liu1,2, Caisheng Huang1,2,3, Xingning Mao2

  • 1Institute of Urology and Nephrology, First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning 530021, Guangxi, China.

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|September 23, 2024
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Summary

Mast cells (MCs) play a crucial role in bladder cancer (BCa) by influencing the tumor microenvironment (TME). Understanding MC-MP interactions offers new avenues for BCa immunotherapy.

Keywords:
Blader CancerMast CellMononuclear PhagocytesSingle Cell RNA-SequencingTumor Microenvironment

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Area of Science:

  • Oncology
  • Immunology
  • Cell Biology

Background:

  • The tumor microenvironment (TME) in bladder cancer (BCa) involves complex cellular interactions.
  • Mast cells (MCs) and Mononuclear Phagocytes (MPs) are key components of the TME, but their specific roles in BCa are not fully understood.

Purpose of the Study:

  • To investigate the interaction between Mast cells (MCs) and Mononuclear Phagocytes (MPs) within the bladder cancer tumor microenvironment (TME).
  • To identify potential immunotherapeutic targets by characterizing MC and MP subtypes and their functions in BCa.

Main Methods:

  • Single-cell RNA sequencing (scRNA-Seq) was performed on samples from 12 BCa patients.
  • Transcriptome data analysis identified MC and MP subgroups, their phenotypes, gene enrichment, cell-cell communication, and biological processes.
  • Cytokine expression (ELISA) and chemotactic effects (Transwell assay) were evaluated.

Main Results:

  • An increase in the interferon-stimulated MC subtype (Mast-ISG15) was observed in muscle-invasive bladder cancer (MIBC).
  • Mast-IL13 and Mast-CCL2 subgroups were enriched in interferon (IFN) and nuclear factor kappa-B (NF-κB) signaling pathways.
  • Activated MCs secreted CCL2 and IL-13, attracting MPs, as confirmed by in vitro experiments.

Conclusions:

  • Mast cells (MCs) significantly shape the tumor microenvironment (TME) in bladder cancer (BCa).
  • These findings provide novel insights into the precise treatment strategies for BCa, highlighting MC-MP interactions as a therapeutic target.