Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Dipeptidyl Peptidase 4 Inhibitors01:23

Dipeptidyl Peptidase 4 Inhibitors

178
Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a...
178
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

496
Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
496
Hormones Regulating Blood Glucose01:16

Hormones Regulating Blood Glucose

3.1K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
3.1K
Oral Hypoglycemic Agents: α-Glucosidase Inhibitors01:19

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

167
α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...
167
Glucose Homeostasis: Regulation of Blood Glucose01:02

Glucose Homeostasis: Regulation of Blood Glucose

1.5K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
1.5K
Overview of Carbohydrate Metabolism01:19

Overview of Carbohydrate Metabolism

792
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
792

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Roles of megakaryocytes and platelets in myeloproliferative neoplasms.

Current opinion in hematology·2026
Same author

Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signaling.

Frontiers in molecular biosciences·2026
Same author

Potential Role of Polyphenols in Platelet Aggregation and Blood Coagulation.

Journal of cardiovascular development and disease·2026
Same author

Fibrin formation and lysis in hyperglycaemia - molecular and cellular drivers.

Current opinion in hematology·2026
Same author

Proteomic profiling identifies systemic drivers of blood-brain barrier injury in sickle cell disease.

Blood·2026
Same author

Enhancing validation of case-control omics signatures through "minimalist" single-subject analysis (N-of-1 trials): proof of concept in sepsis.

Journal of the American Medical Informatics Association : JAMIA·2026

Related Experiment Video

Updated: Jun 12, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

405

Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia.

Julia S Gauer1, Abigail Ajanel2,3, Lutale M Kaselampao1

  • 1Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom.

Research and Practice in Thrombosis and Haemostasis
|September 23, 2024
PubMed
Summary
This summary is machine-generated.

Polyphenols can reduce platelet hyperactivity caused by high blood sugar. These compounds may offer benefits for preventing blood clots in individuals with diabetes.

Keywords:
bioenergeticsclot structurediabeteshyperglycemiaoxidative stressplant-derivedplateletspolyphenols

More Related Videos

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11:10

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Published on: November 16, 2011

94.3K
A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia
09:35

A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia

Published on: November 15, 2024

326

Related Experiment Videos

Last Updated: Jun 12, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

405
Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11:10

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Published on: November 16, 2011

94.3K
A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia
09:35

A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia

Published on: November 15, 2024

326

Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Metabolic Health

Background:

  • Polyphenols are known to reduce oxidative stress and influence glycemic control.
  • The impact of polyphenols on platelet bioenergetics and clot structure in diabetes and hyperglycemia remains unclear.

Purpose of the Study:

  • To investigate how polyphenols affect human platelet bioenergetics.
  • To determine the subsequent impact on clot structure under normoglycemic versus acute hyperglycemic conditions in vitro.

Main Methods:

  • Isolated human platelets were exposed to normoglycemic (5 mM glucose) or hyperglycemic (25 mM glucose) conditions.
  • Platelets were treated with four polyphenols (resveratrol, hesperetin, EGCG, quercetin) to assess effects on ROS, mitochondrial function, and procoagulant activity.
  • Clot structure was analyzed using confocal microscopy for fibrin density and porosity.

Main Results:

  • Acute hyperglycemia elevated platelet reactive oxygen species (ROS), mitochondrial calcium flux, and procoagulant platelet numbers.
  • Resveratrol, quercetin, and EGCG decreased platelet ROS.
  • Resveratrol and EGCG reduced mitochondrial calcium flux in hyperglycemia; resveratrol also decreased procoagulant platelets and attenuated oxygen consumption.

Conclusions:

  • Polyphenols can mitigate the heightened platelet activity associated with hyperglycemia.
  • These findings suggest potential therapeutic applications for polyphenols in thrombosis prevention strategies for diabetic patients.