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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
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Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes

U R Rapp, J L Cleveland, K Brightman

    Nature
    |October 3, 1985
    PubMed

    Insights

    Avian v-myc oncogene expression abrogated growth factor dependence in hematopoietic cells. This oncogene suppressed c-myc expression, impacting cell growth regulation and differentiation.

    Area of Science:

    • Molecular Biology
    • Oncology
    • Cell Biology

    Background:

    • Oncogenes play a role in cell transformation by influencing growth factor signaling pathways.
    • Platelet-derived growth factor (PDGF) induces c-myc and c-fos, genes associated with fibroblast competence.
    • c-myc induction is critical, as its regulated expression can reduce fibroblast dependence on PDGF.

    Purpose of the Study:

    • To investigate the impact of oncogenes on hematopoietic and lymphoid cell differentiation, immortalization, and growth factor dependence.
    • To examine the effects of avian v-myc oncogene expression in hematopoietic/lymphoid cells.

    Main Methods:

    • Utilized recombinant murine retroviruses to express the avian v-myc oncogene.
    • Studied the effects on interleukin-3 (IL-3)-dependent and interleukin-2 (IL-2)-dependent cell lines.

    Main Results:

    • Recombinant avian v-myc expression abrogated the requirement for growth factors (IL-3 or IL-2) in dependent cell lines.
    • v-myc expression led to the suppression of endogenous c-myc expression in these cells.

    Conclusions:

    • The avian v-myc oncogene can override the need for specific growth factors in hematopoietic/lymphoid cells.
    • v-myc's ability to suppress c-myc suggests a complex regulatory role in cell proliferation and differentiation.

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