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Updated: Jun 12, 2025
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
Published on: April 21, 2022
Eight types of RNA modification regulators define clinical outcome and immune response in gastric cancer
Danhong Dong1, Pengfei Yu1, Xin Guo1
1Department of Digestive Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, Shaanxi, China.
Background:
RNA modifications represent a novel category of biological molecule alterations, characterized by three primary classes of proteins: writers, erasers, and readers. Numerous studies indicate that the dysregulation of these RNA modifications is linked to cancer development and may offer new therapeutic avenues for treatment. In our research, we focused on eight specific genes associated with RNA modifications (RMRGs) to comprehensively analyze their distinct functions in gastric cancer (GC). Furthermore, we aimed to elucidate the roles of RMRGs concerning clinicopathological characteristics, tumor microenvironment, and patient prognosis.
Methods:
In this study, we examined the expression and mutations of RMRGs in gastric cancer (GC) using data from TCGA-STAD (The Cancer Genome Atlas; Stomach adenocarcinoma) and the gene expression omnibus (GSE66229). We identified two subtypes of RMRGs and three gene clusters through consensus clustering analysis, assessing their differences in prognosis and immune cell infiltration patterns. Subsequently, we developed an RMRGs score to evaluate GC prognosis and highlight general immune features within the tumor microenvironment (TME). Lastly, we focused on MAMDC2 to validate its expression in GC and explore the effects of a MAMDC2 inhibitor on GC tumor cells.
Results:
We discovered 94 differentially expressed RMRGs common to both the TCGA-STAD and GEO datasets. Notable differences in prognosis and immune cell infiltration were observed between the two RMRGs subtypes and three gene clusters. The RMRGs score emerged as an independent prognostic factor related to the tumor microenvironment (TME) characteristics in gastric cancer (GC). Reducing MAMDC2 levels enhanced cell migration and invasion while decreasing proliferation in vitro.
Conclusions:
In conclusion, this study comprehensively analyzed the role of RMRGs on GC. Our study firstly proposed RMRGs score and demonstrated its potential to be biomarkers for prognosis and immune characteristics. Consequently, RMRGs score is of great clinical significance and can be utilized to develop individualized.
Insights
RNA modification genes (RMRGs) play a role in gastric cancer (GC) development. A novel RMRGs score can predict patient prognosis and tumor microenvironment immune features, aiding personalized treatment strategies.
Area of Science:
- Molecular Biology
- Cancer Research
- Genomics
Background:
- RNA modifications are crucial regulators of biological processes.
- Dysregulation of RNA modification genes (RMRGs) is implicated in gastric cancer (GC) pathogenesis.
- Understanding RMRGs' roles offers potential therapeutic targets for GC.
Purpose of the Study:
- To analyze the functions of eight RMRGs in gastric cancer.
- To investigate the association of RMRGs with clinicopathological features, tumor microenvironment (TME), and prognosis in GC.
- To develop a prognostic and immune-related biomarker for GC.
Main Methods:
- Expression and mutation analysis of RMRGs in GC using TCGA-STAD and GEO datasets.
- Consensus clustering to identify RMRG subtypes and gene clusters.
- Development of an RMRGs score for prognostic evaluation and TME analysis.
- In vitro validation of MAMDC2's role in GC cells.
Main Results:
- Identified 94 differentially expressed RMRGs.
- Observed significant differences in prognosis and immune cell infiltration between RMRG subtypes and clusters.
- The RMRGs score is an independent prognostic factor associated with TME characteristics in GC.
- Reduced MAMDC2 expression promoted GC cell migration and invasion while inhibiting proliferation.
Conclusions:
- RMRGs play a significant role in gastric cancer.
- The proposed RMRGs score serves as a potential biomarker for GC prognosis and immune characteristics.
- The RMRGs score holds clinical significance for developing individualized GC treatments.
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