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$X-ray Diffraction of Biological Samples$ 01:10

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According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...

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Updated: Jun 12, 2025

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

Ultrabroadband Optical Diffraction Tomography.

Martin Hörmann¹, Franco V A Camargo², Niek F van Hulst^3,4

¹Dipartimento di Fisica, Politecnico di Milano, Piazza L. da Vinci 32, Milano 20133, Italy.

|September 23, 2024

Summary

This summary is machine-generated.

We developed ultrabroadband optical diffraction tomography (ODT) for 3D imaging. This technique achieves 150 nm resolution and measures broadband refractive index changes, enabling precise nanoparticle identification in cells.

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Area of Science:

Biophysics
Optical Imaging
Spectroscopy

Background:

Optical diffraction tomography (ODT) is a valuable 3D imaging method.
Integrating ODT with broadband light sources presents significant experimental challenges.
Measuring broadband refractive index changes in 3D samples is difficult with current techniques.

Purpose of the Study:

To introduce ultrabroadband ODT (UB-ODT) for enhanced 3D imaging.
To enable measurement of broadband refractive index variations in samples.
To achieve high lateral spatial resolution for detailed cellular analysis.

Main Methods:

Utilized pulse-shaping-based Fourier transform spectroscopy.
Developed an ODT system with over 150 nm visible spectral bandwidth.
Applied spectrally resolved imaging to biological samples.

Main Results:

Achieved a lateral spatial resolution of 150 nm.
Acquired broadband, spectrally resolved ODT images of HeLa cells.
Demonstrated precise 3D identification of nanoparticles within cellular structures using spectral data.

Conclusions:

UB-ODT overcomes limitations of traditional ODT for broadband analysis.
The technique allows for detailed characterization of refractive index in 3D.
This advancement has significant implications for life sciences and materials science research.