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Related Experiment Videos

Intestinal lactase synthesis during postnatal development in the rat.

M M Jonas, R K Montgomery, R J Grand

    Pediatric Research
    |September 1, 1985
    PubMed
    Summary

    This study investigated the decline in intestinal lactase activity after weaning in rats. Findings suggest reduced lactase synthesis, not structural changes, causes this developmental decrease.

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    Changing genes; losing lactase.

    Gut·2003

    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Gastroenterology

    Background:

    • Intestinal lactase activity significantly declines after weaning in many mammals.
    • The precise mechanism behind this developmental decrease in lactase enzyme activity remains incompletely understood.

    Purpose of the Study:

    • To elucidate the mechanism responsible for the developmental decline in intestinal lactase activity observed at weaning.
    • To investigate the role of lactase synthesis rates in this age-related decrease in enzyme function.

    Main Methods:

    • Purification of lactase from newborn rat intestines and generation of a monospecific antibody.
    • Development of a quantitative immunoprecipitation assay to measure lactase levels.
    • Intraluminal pulse-chase labeling with 3H-leucine in suckling (15-day) and adult rats to quantify newly synthesized lactase within microvillus membranes.

    Main Results:

    • A significantly higher proportion of newly synthesized microvillus membrane protein was identified as lactase in suckling rats compared to adults.
    • Quantitative immunoprecipitation revealed a greater relative rate of lactase synthesis in suckling animals.
    • SDS-polyacrylamide gel electrophoresis and fluorography showed no structural differences between lactase synthesized in suckling and adult rats.

    Conclusions:

    • The developmental decline in intestinal lactase activity at weaning is primarily attributed to a decrease in the rate of lactase synthesis.
    • Changes in the synthesis rate, rather than alterations in enzyme structure, play a crucial role in postweaning lactase activity reduction.

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