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Updated: Jun 12, 2025

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down
Guijie Zhu1, Seyed Amirhossein Sadeghi1, Morteza Mahmoudi2
1Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, USA. lsun@chemistry.msu.edu.
Abstract:
The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine.

