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Published on: May 27, 2016
Circulating exosomal miRNA-451 as an effective diagnostic biomarker and prognostic indicator for multiple myeloma
Jun Zhang1, Cheng Luo1, Haiying Long1
1Department of Hematology, the Second Affiliated Hospital of Guizhou Medical University, No. 3 Kangfu Road, Kaili City, Guizhou Province, 556000, China.
Objective:
Multiple myeloma (MM) is a plasma cell malignancy characterized by abnormal plasma cell proliferation in the bone marrow. Circulating exosomal miRNA-451 is associated with the progression of many tumors, but the relationship between its expression and MM has not been reported. In this study, we aimed to investigate the clinical value of miRNA-451 as a biomarker for diagnosis and prognosis of multiple myeloma.
Methods:
A total of 120 patients with multiple myeloma and 120 healthy control people were recruited in this study. The miRNA-451 expression in serum exosomes of participants was measured by quantitative real-time polymerase chain reaction, and the diagnostic value of miRNA-451 for multiple myeloma was assessed by receiver operating characteristic (ROC) curve. The correlation between miRNA-451 expression and plasma cells ratio and M protein content was analyzed by Pearson correlation coefficient. The prognosis of different miRNA-451 expression was evaluated by survival curves.
Results:
Results suggested that serum exosomal miRNA-451 expression was significantly decreased in patients with multiple myeloma rather than in the healthy controls. The ROC curve showed that area under the curve value of miRNA-451 was 0.888, suggesting that miRNA-451 had diagnostic value to multiple myeloma. Moreover, there was a negative correlation between miRNA-451 expression and plasma cells ratio or M protein content. Survival curves showed that patients with high miRNA-451 expression had a longer survival time, suggesting the value of miRNA-451 as a prognostic indicator of multiple myeloma.
Conclusion:
We demonstrated the relationship between miRNA-451 expression and multiple myeloma, indicating that miRNA-451 in circulating exosomes may be an effective diagnostic biomarker and prognostic indicator for multiple myeloma.
Insights
Serum exosomal miRNA-451 is significantly decreased in multiple myeloma (MM) patients. This biomarker shows diagnostic and prognostic value for MM, with lower levels correlating to disease progression and higher levels indicating longer survival.
Area of Science:
- Biochemistry
- Oncology
- Molecular Biology
Background:
- Multiple myeloma (MM) is a plasma cell malignancy.
- Circulating exosomal microRNA-451 (miRNA-451) is implicated in various tumor progressions.
- The role of exosomal miRNA-451 in MM remains unelucidated.
Purpose of the Study:
- To investigate the clinical utility of exosomal miRNA-451 as a diagnostic and prognostic biomarker for multiple myeloma.
- To determine the correlation between exosomal miRNA-451 expression and MM disease indicators.
Main Methods:
- Quantitative real-time polymerase chain reaction was used to measure serum exosomal miRNA-451 levels in 120 MM patients and 120 healthy controls.
- Receiver operating characteristic (ROC) curve analysis assessed diagnostic value.
- Pearson correlation coefficient and survival curve analyses evaluated prognostic significance.
Main Results:
- Serum exosomal miRNA-451 expression was significantly lower in MM patients compared to healthy controls.
- ROC analysis yielded an area under the curve of 0.888, indicating diagnostic potential.
- Decreased miRNA-451 levels correlated with higher plasma cell ratios and M protein content.
- Higher miRNA-451 expression was associated with longer patient survival.
Conclusions:
- Exosomal miRNA-451 is a potential diagnostic biomarker for multiple myeloma.
- Circulating exosomal miRNA-451 serves as a valuable prognostic indicator for MM patients.
- This microRNA may play a role in MM pathogenesis and progression.
