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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
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Syphilitic lymphadenitis: immunofluorescent identification of spirochetes from imprints

Y J Choi, L Reiner

    The American Journal of Surgical Pathology
    |December 1, 1979
    PubMed

    Abstract:

    We are reporting two cases of early syphilis with inguinal buboes which were surgically excised. Th clinical impression was lymphoma in one case and inguinal hernia in the other. The correct and specific diagnosis was quickly established by the application of fluorescent antibody technique to imprints of the enlarged lymph node.

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    Immunofluorescence Microscopy01:12

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    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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