Related Experiment Video

Updated: Jun 12, 2025

Author Spotlight: Optimizing Grid Preparation for Enhanced Cryoelectron Tomography
08:17

Author Spotlight: Optimizing Grid Preparation for Enhanced Cryoelectron Tomography

Published on: December 15, 2023

3.0K

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining

Philip Seifert1

  • 1Morphology Core, Schepens Eye Research Institute of Mass Eye and Ear Boston, Boston, MA, USA.

Journal of Histotechnology
|September 23, 2024
PubMed

Keywords:
3D printerAutomated stainerHiraokaHistoEnderlead staintransmission electron microscopy griduranyl acetate

More Related Videos

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition08:01

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

Published on: December 22, 2011

58.7K
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach04:51

Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach

Published on: December 29, 2023

5.3K

Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K