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Pseudomonas aeruginosa Mediates Host Necroptosis through Rhl-Pqs Quorum Sensing Interaction
Zihao Liu1,2, Lu Sun3, Lupeng Li4,5,6,7
1Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH.
Immunohorizons
|September 23, 2024
Summary
Pseudomonas aeruginosa quorum sensing (QS) regulates host cell death. A deficiency in the rhl QS system increases macrophage necroptosis, mediated by the pqs system, revealing a key cross-talk during infection.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen.
- Bacterial quorum sensing (QS) regulates virulence and host interactions.
- The role of P. aeruginosa QS in host programmed cell death remains unclear.
Purpose of the Study:
- To investigate the role of P. aeruginosa QS in host programmed cell death.
- To elucidate the specific QS systems involved in P. aeruginosa-induced cell death.
Main Methods:
- Utilized P. aeruginosa mutants deficient in QS systems (rhl, pqs).
- Assessed macrophage cell death and necroptosis markers (RIPK3, MLKL phosphorylation) in vitro and in vivo.
- Analyzed the interplay between rhl and pqs QS systems.
Main Results:
- Mice bone marrow macrophage cell death increased in P. aeruginosa rhl-deficient infections.
- Elevated RIPK3 and MLKL phosphorylation indicated heightened necroptosis.
- Upregulation of the pqs QS system by the rhl deficiency caused necroptosis.
- Deletion of pqs in rhl-deficient P. aeruginosa abolished macrophage necroptosis.
Conclusions:
- P. aeruginosa QS significantly influences host necroptosis.
- The rhl-pqs QS axis mediates P. aeruginosa-induced macrophage necroptosis.
- Highlights cross-talk between bacterial QS and host immune pathways.
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