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Injectable calcium phosphate cement integrated with BMSCs-encapsulated microcapsules for bone tissue regeneration
Yafei Yuan1, Jiangqi Hu1, Lipei Shen1
1Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing 100050, People's Republic of China.
Biomedical Materials (Bristol, England)
|September 23, 2024
Summary
Injectable calcium phosphate cement (CPC) enhanced with alginate-chitosan-alginate (ACA) microcapsules loaded with stem cells improves bone defect repair. The microcapsules support cell viability and promote bone regeneration by recruiting endogenous cells.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Injectable calcium phosphate cement (CPC) is beneficial for bone defect repair but lacks osteogenic properties and sufficient porosity.
- Addressing these limitations is crucial for enhancing bone regeneration efficacy.
Purpose of the Study:
- To investigate the use of alginate-chitosan-alginate (ACA) microcapsules encapsulating rat bone mesenchymal stem cells (rBMSCs) to improve CPC performance.
- To evaluate the osteogenic potential and bone regeneration capacity of ACA-CPC composites in a rat cranial defect model.
Main Methods:
- ACA microcapsules were fabricated to encapsulate rBMSCs for delivery into CPC paste.
- A porous CPC scaffold was created to support cell growth.
- The composite material was implanted into rat cranial parietal bone defects.
- Cell viability, osteogenic differentiation, and bone formation were assessed.
Main Results:
- ACA microcapsules effectively protected rBMSCs and enhanced their viability post-implantation.
- ACA + CPC extracts promoted osteogenic differentiation of rBMSCs.
- The composite material improved in situ osteogenesis and bone growth in defects.
- Microcapsule-induced pores facilitated ongoing bone formation by recruiting endogenous cells.
Conclusions:
- ACA-loaded stem cell microcapsules enhance the osteogenesis and degradation of CPC.
- This approach represents a promising strategy for bone defect transplantation and regeneration.
