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[Neutralizing antibodies to human gamma-interferon].
Voprosy Virusologii
|May 1, 1985
Summary
Researchers developed a method to produce gamma-interferon from spleen lymphocytes. This process yielded a partially purified interferon and a specific neutralizing antibody in rabbits, crucial for interferon research.
Area of Science:
- Immunology
- Virology
- Biochemistry
Context:
- Human spleen lymphocytes are a source of gamma-interferon.
- Staphylococcal enterotoxins A and B effectively induce gamma-interferon production.
- Gamma-interferon can be purified using porous glass and gel chromatography.
Purpose:
- To establish a method for producing and purifying gamma-interferon from human spleen lymphocytes.
- To induce the production of a neutralizing antibody against gamma-interferon in rabbits.
Summary:
- Human spleen lymphocytes were cultured and stimulated with staphylococcal enterotoxins A and B to produce gamma-interferon (IFN-γ).
- A partially purified IFN-γ preparation with an activity of 2 X 10(4) units/ml was achieved through porous glass sorption and Sephadex G-25 chromatography.
- Rabbits immunized with this preparation developed a specific neutralizing antibody against gamma-interferon.
Impact:
- This work provides a method for obtaining purified gamma-interferon and its specific antibody.
- The developed antibody demonstrates high specificity, showing no cross-reactivity with other human interferon types (alpha and beta).
- This resource is valuable for further research into gamma-interferon's biological functions and therapeutic applications.